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Effects of Pilocarpine Hydrochloride on the Hyposalivation Induced by Psychotropic Drugs



Author(s)

Júlia Ribas César Durscki, Aline Cristina Batista Johann, Antônio Adilson Soares Lima, Maria Ângela Naval Machado, Luciana Reis Azevedo, Edvaldo Antonio Ribeiro Rosa and Ana Maria Trindade Grégio

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DOI:10.17265/1934-7391/2013.12.003

The aim was to study the secretagogue action of pilocarpine on the murine parotid glands submitted to chronic treatment with psychotropic drugs by salivary flow rate determinations and histological alterations. Fifty four male Wistar rats were equally divided in three groups: C group (control) received saline solution for 30 days; AD group (n = 18) received Amitriptyline^® and Diazepam^® for 30 days, and ADP group (n = 18) received Amitriptyline^® and Diazepam^® for 30 days and Amitriptyline^®, Diazepam^® and pilocarpine for further 30 days, resulting in 60 days of treatment. Saliva samples were collected 30 h after the end of treatment. Parotids were removed and processed for hematoxylin-eosin histological analysis. Dedicated software for image processing allowed the determination of cell number and volume. Significant differences between paired-groups C-AD (P < 0.01) and AD-ADP (P < 0.01) were observed for glands size and weight. The volume of serous cells was greater in AD, suggesting a hypertrophy of the salivary glands. For salivary flow rate, C group showed the highest average. The number of serous cells was similar between groups ADP and C, with the lowest average being found in AD group (P < 0.05).

September 11, 2013 / Accepted: December 05, 2013 / Published: December 30, 2013.