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Optimization on Pretreatment and Enzymatic Hydrolysis of Sugarcane Trash for Ethanol Production



Author(s)

Teerapatr Srinorakutara1, Suthkamol Suttikul1, Ekarat Butivate2, Vishnu Panphan1 and Nassapat Boonvitthya3

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DOI:10.17265/2159-5828/2014.03.004

1. Department of Energy Technology, Thailand Institute of Scientific and Technological Research (TISTR), Pathum Thani 12120, Thailand
2. Department of Agricultural Technology, Thailand Institute of Scientific and Technological Research (TISTR), Pathum Thani 12120, Thailand
3. PTT Research and Technology Institute, PTT Public Company Limited, Ayutthaya 13170, Thailand

The present study was conducted for the optimization of pretreatment and enzymatic hydrolysis of lignocellulosic biomass (sugarcane trash), which is a renewable resource for the production of bioethanol. The pretreatment and enzymatic hydrolysis conditions including alkali (NaOH)/dilute acid (H₂SO₄), substrate and chemical concentration for pretreatment, enzyme dosage, pH, temperature and substrate concentration for hydrolysis were varied and evaluated for sugar and ethanol production at the end. The optimum condition was accomplished using 15% w/v DS of 0-2 mm sugarcane trash in size of particle. It was pretreated with two steps of 2% w/v NaOH autoclaving followed by 2% w/v H₂SO₄ autoclaving with washing step after pretreatment. An enzymatic hydrolysis was then performed using 15% w/v DS pretreated substrate, hydrolyzed with cellulase 50 filter paper unit (FPU)/g DS at 50 °C and pH 5. After incubating at 160 r for 48 h, 117.16 g/L reducing sugar was obtained. The achieved sugar after enzymatic hydrolysis was finally fermented to ethanol by Saccharomyces cerevisiae TISTR 5596, with concentration of 48.17 g/L ethanol or yield 0.509 g/g reducing sugars which was equal to 99.81% of theoretical yield.

Cellulosic biomass, pretreatment and enzymatic hydrolysis, sugarcane trash, ethanol production.