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Characterization of Lead (II)—Tris Complex



Author(s)

David Gvarjaladze¹, Shota Gogichaishvili², Levan Lomidze¹ and Nunu Metreveli¹

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DOI:10.17265/2161-6213/2026.4-6.004

1. Institute of Biophysics, Ilia State University, 45 Ilia Chavchavadze Avenue, Tbilisi 0177, Republic of Georgia
2. Andronikashvili Institute of Physics, I. Javakhishvili Tbilisi State University, Tamarashvili Street 6, Tbilisi 0177, Republic of Georgia

Tris, or known during medical use as tromethamine or THAM (tris (hydroxymethyl) aminomethane), is an organic compound with the formula NH₂C (CH₂OH)₃. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE (Tris, Acetic acid and EDTA (Ethylenediaminetetraacetic acid)) and TBE (Tris, Boric acid and EDTA (Ethylenediaminetetraacetic acid)) buffers, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes. Tris also complexes with metal ions in solution. Lead (II) chloride (PbCl₂) is an inorganic compound which is a white solid under ambient conditions. Lead (II) chloride is one of the most important lead-based reagents. In this research the tris -Pb²⁺ complex was studied using a spectrophotometer. The study showed that complex is optical active and Pb²⁺ coordinate a thermally stable complex with Tris buffer.

Absorption spectra, Lead (II) chloride, spectroscopy.