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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Astija
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DOI:10.17265/2161-6256/2017.06.004
Affiliation(s)
Universitas Tadulako, Kemenristekdikti 94119, Indonesia
ABSTRACT
Sucrose is a sugar required for pollen germination and pollen tube
elongation. It is little known on the regulation mechanism. As such, this research was conducted to reveal mechanism pathway of the sugar in
regulating pollen germination and pollen tube elongation by cell wall
invertase. The pollen grains, respectively originated
from wild type and transgenic tomato
plants, which had been silenced
their inhibitor gene (INVINH1) of the cell wall invertase were used in this
study. The pollen grains were cultured in media
containing glucose, fructose or sucrose. Results of the study showed that percentage of the pollen germination
derived from transgenic plants was much higher than that from wild type plants.
Moreover, pollen tube elongation was longer in transgenic plants compared with
wild type plants. Interestingly, these results were observed in medium
containing sucrose only, but not in glucose
and fructose. This result suggests that cell wall invertase activity regulates
pollen germination and pollen tube elongation through sucrose hydrolysis.
KEYWORDS
Sucrose, cell wall invertase, pollen germination, pollen tube elongation, tomato.
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