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Isolation of Main Genes Involved in Flowering of Saffron (Crocus sativus L.) in Order to Study Effective Flowering Protein



Author(s)

Iman Yousefi Javan and Yousef Hosseinzadehgonabad

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DOI:10.17265/2159-5828/2017.08.003

Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat-e Heydarieh 9516168595, Iran

Saffron (Crocus sativus L.) always is grown for using its flowers in nutrient industry, color industry and healthy compounds due to its flowers and specially stigmas. Because of its expensive flowers, surveying and recognizing on effective genes for flowering is very important and its results can help us to control rate and timing of flowering at an early stage of flowering. The gene and gene state meant Pistillata like MADS box (PIC2) were surveyed for recognizing its molecular mechanism. The molecular sequence at the genes has high similarity to members of family MADS that is a factor for controls of protein at flowering stage. PIC2 gene was studied by bioinformatics resources. Primers were designed for replicating the gene and DNA and RNA were extracted from saffron’s leaves. The gene’s cDNA was built by recopying enzyme and used such a pattern for replicating gene PIC2 at polymerase chain reactions (PCR). Segments were replicated such 900 cDNA pair-nucleotides and a segment such 2,100 of DNA’s pair-nucleotides. The gene codes a protein that was composed of 210 amino acids that has MADS sequence box. Analysis of protein’s molecular structure and homological modeling of the protein indicated that it has a regular structure.

Saffron, PIC2 gene, MADS sequence box, gene state, homological modeling.