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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Staining Effect of Pomegranate Flower Extract on Human Blood Cells: First Results
Author(s)
Nilgun Guler Kusculuo and Huseyin Benli
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DOI:10.17265/2162-5298/2017.05.004
Affiliation(s)
Department of Chemistry Technology, Vocational School of Mustafa Cıkrıkcıoglu, University of Erciyes, Kayseri 38039, Turkey
ABSTRACT
Pomegranate flower plant, popularly known using for the treatment of
various diseases, was not investigated
as a source of dye for cytological studies using human blood cells. The
importance of this study is to appear dyeing result of pomegranate flower
extract on human blood cells. The natural dye source was pomegranate flower
known as roselle and potassium
aluminum sulfate (alum = KAlSO412H2O) was used as mordant
or metal salt. Distilled
water was used as
solvent. Fresh, clean and air-dried flowers were extracted with distilled water at 100 °C for 30 minutes and then filtered.
One drop blood from a healthy 20-year woman was spread
as a peripheral on to ten plates and dried at nearly 25 °C. These slides were stained by soaking in pomegranate flower extract with/without
alum
(KAlSO412H2O)
at 100 °C for 60 minutes. Slides were washed with distilled
water, dried and done microscobic examination. The different blood cells dyed
dark orange in alum mordant media at 100 °C. As a result, pomegranate flower has the capacity to use dyeing human
blood cells such as eosinophil, basophil and neutrophil.
KEYWORDS
Blood, neutrophil, pomegranate flower, basophil, eosinophil.
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