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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Dilek Yalçin Duygu
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DOI:10.17265/2162-5263/2017.04.003
Affiliation(s)
Department of Biology Education, Gazi University, Ankara 06530, Turkey
ABSTRACT
Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae
on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chosen among 11 C. vulgaris strains isolated from different
fresh water ponds and their cell numbers and the amount of chlorophyll a, protein,
lipid, cellulose and carbohydrate were examined. The main goal of the study is to
investigate increasing the biochemical contents
especially the protein content of C. vulgaris strains in different mediums. In the present study, cell
densities were determined through cell count for five days. In parallel with cell
count, their chlorophyll a content was determined. The highest cell density was observed with C. vulgaris TOH (Tourism
and Hotel Management Pond) strain as 5.5 × 104 h/mL, and the chlorophyll a content as 4.3 × 102 mg/m3.
The highest intracellular protein amount was determined with C. vulgaris GUH (Gazi University
Rectorship Pond) (0.061 g/100 mL) and the highest lipid
amount was attained with C. vulgaris UIK (Ulus Construction Well) strain as 0.019 g/100 mL. The process of increasing
the intracellular protein amount in C. vulgaris GUH strain was carried
out in Prat, Yagojinski and Chlorella medium.
The results indicated that Chlorella medium increased the
intracellular protein amount.
KEYWORDS
Chlorella vulgaris, chlorophyll a, microalgae, biomass, biochemical analysis.
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