Contact us
 |
[email protected] |
 |
3275638434 |
 |
 |
| Paper Publishing WeChat |
Useful Links
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Mohammad Waleed Khudhair, Hussain Fadhel Alrubeai and Mohammed Zaidan Khalaf
Full-Text PDF
XML 1096 Views
DOI:10.17265/2161-6256/2016.06.004
Affiliation(s)
Integrated Pest Control Research Center, Directorate of Agricultural Research, Ministry of Science and Technology, P.O. Box 765, Baghdad, Iraq
ABSTRACT
The main objective of this study was to investigate the
presence of natural endophytic Beauveria bassiana within date palm
tissues using molecular technique and measure their field efficacies in controlling Dubas bug, Ommatissus lybicus (Deberg). Two entomopathogenic B. bassiana isolates (MARD 108 and 100) were isolated from
date palm, Phoenix dactylifera L. leaves; in addition, one isolate (MARD
92) originally from soil was identified to have endophytic property. Concentration of 1 × 109 conidia/mL of each of three endophytic isolates was used in field experiments
targeting Dubas bug nymphs via injection tree trunks. The results indicated that the high mortality
rates reached 92%, 96% and 100% with infliction of the three
endophytic isolates after 15 d from the treatment. The successful establishment
of the fungal isolates in the date palm tissue was determined using B. bassiana species-specific primer for
the first time via using conventional polymerase chain reaction (PCR)
amplification technique before and after injection, and the positive gel band representation was the
identification signs. The novel results depicted for the first time the
presence of natural endophytic B. bassiana isolates within date palm
tissues and their field efficacies in controlling Dubas bug, O. lybicus (Deberg) infestation.
KEYWORDS
Dubas bug, Ommatissus lybicus, Beauveria bassiana, endophytes, date palm orchards.
Cite this paper
References