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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Motility of Stallion Spermatozoa after Centrifugation and Cooling in INRA96® or Walworth Extender
Author(s)
Gabrielle Nicolette Riccio1, Robyn Ellienne Ellerbrock2, Igor Frederico Canisso2, Robert Victor Knox1 and Kevin Halden Kline1
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DOI:10.17265/2161-6256/2016.02.008
Affiliation(s)
1. Department of Animal Sciences, University of Illinois Urbana-Champaign, 1207 West Gregory Drive, Urbana, IL 61801, USA
2. Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA
ABSTRACT
A total of 18 ejaculates
were collected, once per week, from six fertile stallions for three consecutive weeks in
October and November, to compare motility over time between extenders using
four semen processing treatments. Four total aliquots of semen were used. Two
aliquots of each semen collection were extended in either INRA96® or an
experimental proprietary milk-based extender Walworth (WW) extender, and each was designed for
multi-day storage of fresh chilled semen. Each aliquot was divided again, and
either centrifuged at 600 ×g for 10 min
without cushion, or not centrifuged and extended to a
final concentration of 25 × 106 spermatozoa/mL. The treatments
evaluated were INRA96® without centrifugation (INRA-NC) or with centrifugation
(INRA-C), and Walworth extender without centrifugation (WW-NC) or with
centrifugation (WW-C). Total and progressive motility were measured using Sperm
Vision® CASA at 0, 24, 48 and 72 h post-collection. Samples were stored at 4 °C. No differences were found between extenders in progressive
(P = 0.13) or total motility (P = 0.14) over the four different time
points without centrifugation. However, ejaculates processed in INRA-C group had the greater total and
progressive motility (P < 0.05) over the four
time points than ejaculates in the WW-C group. It was found that centrifugation
and re-suspension of stallion semen in INRA96® improved the longevity of fresh
chilled semen. However, when not using centrifugation, the Walworth extender
proved to be as effective for maintaining spermatozoa motility across all time
points as INRA96®,
and may be an alternative for use in the equine breeding industry.
KEYWORDS
Stallion, semen, extender, centrifugation, fresh chilled.
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