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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Teerapatr Srinorakutara1, Yuttasak Subkaree1, Nassapat Boonvitthya2, Thapparait Kunhanon1 and Nantana Bamrungchue1
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DOI:10.17265/2159-5828/2015.02.003
Affiliation(s)
1. Department of Energy Technology, Thailand Institute of Scientific and Technological Research (TISTR), Pathum Thani 12120, Thailand
2. PTT Research and Technology Institute, PTT Public Company Limited, Ayutthaya 13170, Thailand
ABSTRACT
Effect of commercial cellulose enzymes was investigated by batch enzymatic hydrolysis at 15.0% (w/v) solid. It was found that the best
commercial cellulose enzyme was Cellic® CTec comparing to Accellerase
1000TM and Accelerase 1500TM. The Cellic® CTec gave the highest
reducing sugar concentration and rice straw conversion. Moreover, when the hydrolysate obtained from hydrolysis using Cellic® CTec was fermented by Saccharomyces
cerevisiae TISTR 5596, it would
give the highest ethanol. In this study, the Cellic® CTec was used for fed-batch
prehydrolysis prior to ethanol production by simultaneous
saccharification and fermentation (SSF) way at 20% (w/v)
solid loading. It could produce
35.76 g/L or 4.6% (v/v) of ethanol
concentration and 83.67 L/ton dry matter (DM) of yield.
KEYWORDS
Rice straw, sulfuric acid, commercial cellulase, pretreatment, fermentation, ethanol.
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