Open    Access



Extract of Curcuma longa L. and (-)-Epigallo Catechin-3-Gallate Enhanced Proliferation of Adipose Tissue–derived Mesenchymal Stem Cells (AD-MSCs) and Differentiation of AD-MSCs into Endothelial Progenitor Cells



Author(s)

Wahyu Widowati, Caroline Tan Sardjono, Laura Wijaya, Dian Ratih Laksmitawati and Ferry Sandra

Full-Text PDF XML     4445 Views

DOI:10.17265/1548-6648/2012.01.003

Objectives: Previous studies reported that proliferation and differentiation of stem cell are influenced by free radicals. Therefore, we conducted an investigation to know whether antioxidants, to our current interest, extract of Curcuma longa L. (ECL) and (-)-Epigallo catechin-3-gallate (EGCG), are playing role in differentiation and proliferation of adipose tissue-derived mesenchymal stem cells (AD-MSCs). Materials and Methods: ECL and AD-MSCs were prepared. Inhibitory concentration-median (IC-₅₀) of ECL and EGCG were measured based on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity. To examine the effect of ECL and EGCG on proliferation and differentiation of AD-MSCs, ECL and EGCG in various concentrations were applied in culture of AD-MSCs for different period of time. Cell number was counted by trypan blue exclusion method. Differentiation of AD-MSCs into endothelial progenitor cells (EPCs) was analyzed based on following surface markers: cluster of differentiation 34 (CD34), CD133 and vascular endothelial growth factor receptor 2 (VEGFR-2) with flow cytometer. Results: IC₅₀ of ECL and EGCG on DPPH scaven ing activity were 7.61 and 0.42 μg/mL, respectively. The highest proliferation rates were achieved by induction of ECL in concentrations of 1 mg/mL, while induction of EGCG in concentration of 0.25 mg/mL. ECL and EGCG enhanced differentiation of AD-MSCs into EPCs, marked by increasing expression of CD34, CD133 and VEGFR-2 at 4 incubation days. Conclusions: Our current results suggested that ECL and EGCG as antioxidant could enhance proliferation of AD-MSCs and differentiation of AD-MSCs into EPCs.

Antioxidant, curcuma longa, EGCG, EPCs, AD-MS, VEGFR-2, CD133, CD34.