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Incorporation of Bromelain into Dermatological Bases: Accelerated Stability Studies



Author(s)

Iara Rocha Antunes Pereira Bresolin, Igor Tadeu Lazzarotto Bresolin, Priscila Gava Mazzola and Elias Basile Tambourgi

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DOI:10.17265/1934-7375/2014.03.009

This work aimed the incorporation of the enzyme bromelain from pineapple peel into dermatological bases for potential therapeutic application. The enzyme was incorporated into Lanette cream and lotion, as well as Carbopol gel and Chemyunion® cream and lotion, at a concentration of 0.5% (w/w). These bases were subjected to centrifugation test and accelerated stability test during 90 days at 25 °C (with and without sunlight), 37 °C and 4 °C, in order to evaluate bromelain stability in dermatological bases. Total protein content and enzymatic activity were measured. The bases were stable as its organoleptic characteristics (appearance, color, smell and sensitivity to touch) only when kept at 4 ºC with activity remaining 95.5%, 84,9%, 77.7%, 73.8% and 72.3%, after 90 days of testing in Carbopol gel, Lanette and Chemyunion® cream, and Lanette and Chemyunion® lotion, respectively. Based on the results, it was possible to incorporate bromelain into dermatological bases, and the activity was well preserved when these bases were kept in refrigerator at 4 ºC.

Emulsions, stability, bromelain, therapeutic applications, enzymatic activity.