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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Effect of Moisture Content on Pollen Storage of Hevea Clone RRIM 600 and RRIT 251
Author(s)
Sayamol Kaewbunjong1and Uraiwan Tongkaemkaew2*
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DOI:10.17265/2161-6221/2015.3-4.008
Affiliation(s)
1. Songkhla Rubber Research Centre, Rubber Research Institute of Thailand, Songkhla 90110, Thailand 2. Faculty of Technology and Community Development, Thaksin University, Phatthalung 93110, Thailand
ABSTRACT
Non-synchronous flowering
and low natural pollination make breeding between clones of Heveabrasiliensis difficult. Pollen storage in liquid nitrogen (-196 ºC) was an ideal strategy for long-term preservation for
hybridization. This study were investigated pollen viability of clone RRIM 600
and RRIT 251 after long storage in liquid nitrogen. Anther of both rubber clone
were dried to 7% and 11% of moisture content levels before storage in
liquid nitrogen. Pollen viability was testing by in-vitro germination in
solution media and 2, 3, 5-triphenyl-tetrazolium-chloridestained. The results
showed the moisture content at 11% storage 180 days were found pollen stained and
pollen germination higher than at 7% of moisture content. This were the pollen
stained 2.00% in clone RRIM 600 and 2.06 % in clone RRIT 251 while pollen
germination were 1.31 % in clone RRIM 600 and 1.38% in clone RRIT. Besides, the
pollen storage for 90 days in both moisture contents having correlated to the
duration of Hevea flowering period were found pollen viability more than
10%. This finding will be the base information on the utilization of breeders
for hybridization of Hevea varieties.
KEYWORDS
Heveabrasiliensis, rubber tree, liquid nitrogen, pollen viability, pollen germination.
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