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Influence of Solvent Polarity and Conditions on Extraction of Antioxidant, Flavonoids and Phenolic Content from Averrhoa bilimbi



Author(s)

Norlia Muhamad, Siti Aishah Muhmed, Mashitah M. Yusoff and Jolius Gimbun

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DOI:10.17265/2159-5828/2014.05.006

This paper presents the influence of solvent polarity and extraction conditions on the extraction of total flavonoid, total phenolic and antioxidants from Averrhoa bilimbi. The experiment was performed using a different solvent at different extraction conditions, including extraction time (15-240 min), temperature (30-70 °C) and agitation speed (50-300 rpm). Results showed that yields of extraction varies with solvent polarity. Extraction using 50% aqueous methanol gives the highest antioxidant activity and flavonoid content. The highest total flavonoid content (193.3 µg quercetin equivalent/g dry weight), total phenolic content (717.8 µg gallic acid equivalent/g dry weight) and antioxidant activity (77%) was achieved using 50% methanol, at 70 °C and agitation speed of 300 rpm. This work may be useful for obtaining higher bioactive compounds during the extraction process of A. bilimbi.

Extraction, flavonoid, belimbing buluh, phenolic, antioxidant.