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Affiliation(s)

1. Department of Chemical Engineering, USP, R. do Lago, São Paulo 250, 05508-080, Brazil
2. Department of Physical, Chemical and Geological Oceanography, USP, Praça do Oceanográfico 191, SP, Brazil
3. Department of Civil and Environmental Engineering, Rice University, 6100 Main St. Houston, Texas 77005, USA

ABSTRACT

The UV irradiation is used for removing Antibiotic Resistant Bacteria (ARB) and Antibiotic Resistance Genes (ARG) from wastewater treatment. Bacteriophages are viruses that infect within bacteria, are recognized for bacterial control. The influence of some parameters in quantification and performance influencing of pathogen demobilization could be considered in disinfection of wastewater. The comparison of Polyvalent phage (NE1) versus Coliphage (NE4) in suppressing a bacterium Escherichia coli (NDM-1: b-lactam-resistant) with UV irradiation was observed the efficacy in reduction of cells in the disinfection and parameter process. The results with the effect of UV-C irradiation on NDM-1 infected with 1 % of NE4 showed a decrease of cells from 8 x 106 to 2 x 105 in 60 min with UV-C dose. The NDM1 (E. coli) was infected with 1 % of NE4 (Polyvalent Phage) under magnetic stirring for 1 h, the cells count was 8 x 106. After 1 h in UV-C exposure, the cells number reached 3 x 105. The NDM1 that was exposed in 1 h of UV-C irradiation and then was infected with 1 % of NE4. Cells counting were done 24 h after this procedure. These cells were exposed in UV-C and showed a reduction in the number of cells from 1 x 108 to 4 x 105 after 60 min. The results indicate that bacteriophages can mitigate bacteria species, and combined the conventional water disinfection technologies that can support the microbial safety control strategies.

KEYWORDS

Antibiotic Resistant Bacteria (ARB), Antibiotic Resistance Genes (ARG), wastewater treatment, disinfection, Escherichia coli (E. coli).

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