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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Mikaila Zi Yue Smith, Anna Dilger, Erin Bryan, Giana Bytnar, Suhna Choi, Kelly Cook and Kevin Kline
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DOI:10.17265/2161-6256/2022.02.003
Department of Animal Sciences, University of Illinois at Urbana Champaign, 1207 West Gregory Drive, Urbana, IL 61801, USA
The study aimed to investigate the effects of centrifugation of stallion semen on progressive sperm motility over a 72-hour period; and determine if the protein marker, precursor of A-kinase anchor protein 4 (pro-AKAP4), may be maintained at a higher level over time when compared to non-centrifuged samples. Pro-AKAP4 has been studied for its function in stabilizing the structure of spermatozoa and its use as a marker for fertilizing capacity of spermatozoa. Semen collections were obtained from four fertile, commercially used Standardbred stallions collected three times each. Semen samples were diluted to a final concentration of 50 million total cells per milliliter. One extended aliquot of semen from each collection was used for the non-centrifuged treatment, and one extended aliquot of semen was centrifuged at 750× g for 10 min. The supernatant from the centrifuged sample was then removed with a 10 mL plastic transfer pipette, and the spermatozoa cell pellet was suspended in fresh extender to a final concentration of 50 million total cells per milliliter. Both centrifuged and non-centrifuged samples from each collection were stored in Equine Express II stallion semen shipping containers for 72 h and fresh ice packs were added to each container every 24 h. Both progressive sperm motility and levels of pro-AKAP4 were determined at times 0, 12, 24, 48 and 72 h after collection. Differences were found between stallions, times and treatments with the main finding being that pro-AKAP4 concentration in centrifuged samples was maintained at a much higher level over the 72-hour period studied.
Centrifuged, extender, pro-AKAP4, spermatozoa, stallion.
Smith, M. Z. Y., Dilger, A., Bryan, E., Bytnar, G., Choi, S., Cook, K. and Kline, K. 2022. "Preliminary Study of Effects of Centrifugation of Stallion Semen on Motility and Pro-AKAP4 Concentration over 72 Hours Post-collection" Journal of Agricultural Science and Technology A 12 (2022): 65-72.
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