 |
[email protected] |
 |
3275638434 |
 |
 |
| Paper Publishing WeChat |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Zineb Boumart, Zahra Bamouh, Noha Semmate, Khalid Omari Tadlaoui and Mehdi El Harrak
Full-Text PDF
XML 1931 Views
DOI:10.17265/2161-6256/2021.01.004
Research and Development Bacteriology, Multi-Chemical Industry, Lot. 157, Z I, Sud-Ouest (ERAC) B.P.: 278, Mohammedia 28810, Morocco
In this study, 55 suspected pasteurellosis clinical cases from different provinces of Morocco were investigated. Molecular analysis revealed that 47% of samples were positive for Pasteurella multocida, all typed as serogroup A, and 11% positive for Mannheimia heamolytica. Eight isolates were recovered from 26 P. multocida positive samples, and characterized by biochemical and molecular typing methods. Among these isolates, two strains (S13 and S14) were selected for genes (RNA16S and rpoB) sequence analysis and virulence study in mice, guinea pigs and sheep. Phylogeny study showed similarities of both S14 and S13 isolates with strains from other species. In laboratory animals, the strain S14 was more virulent than S13 and induced severe illness in sheep. The high mortality of infected mice suggests that this model may represent an alternative for testing pathogenicity and vaccine efficacy.
Pasteurella multocida, phylogeny, mice, sheep, pathogenicity.
Boumart, Z., Bamouh, Z., Semmate, N., Tadlaoui, K. O., and Harrak, M. 2021. "Biological Characterisation and Pathogenicity of a Pasteurella multocida Isolate from Sheep in Morocco." Journal of Agricultural Science and Technology A 11: 53-64.
[1] Angen, Ø., Mutters, R., Caugant, D. A., Olsen, J. E., and Bisgaard, M. 1999. “Taxonomic Relationships of the [Pasteurella] Haemolytica Complex as Evaluated by DNA-DNA Hybridizations and 16s rRNA Sequencing with Proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. Nov.” Int J Syst Bacteriol 49 (1): 67-86.
[2] Blackall, P. J., Bojesen, A. M., Christensen, H., and Bisgaard, M. 2007. “Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov., comb. nov.” Int J Syst Evol Microbiol. 57: 666-74.
[3] Odugbo, M. O., Odama, L. E., Umoh, J. U., and Lombin, L. H. 2004. “The Comparative Pathogenicity of Strains of Eight Serovars and Untypable Strains of Mannheimia haemolytica in Experimental Pneumonia of Sheep.” Vet Res. 35: 661-9.
[4] Fodor, L., Pénzes, Z., and Varga, J. 1996. “Coagglutination Test for Serotyping Pasteurella haemolytica.” J Clin Microbiol. 34: 393-7.
[5] Gonzalez, J. M., Lacasta, D., Ferrer-Mayayo, L. M., Figueras, L., Abadie, G., and Heras, M. 2013 “Mannheimia haemolytica and Bibersteinia trehalosi Serotypes Isolated from Lambs with Ovine Respiratory Complex in Spain.” J Hell Vet Med Soc. 64: 177-82.
[6] Legesse, A., Abayneh, T., Mamo, G., Gelaye, E., Tesfaw, L., Yami, M., et al. 2018. “Molecular Characterization of Mannheimia haemolytica Isolates Associated with Pneumonic Cases of Sheep in Selected Areas of Central Ethiopia.” BMC Microbiol. 18: 205.
[7] Rímac, R., Luna, L., Hurtado, R., Rosadio, R., and Maturrano, L. 2017. “Detection and Genetic Characterization of Pasteurella multocida from Alpaca (Vicugna pacos) Pneumonia Cases.” Trop Anim Health Prod. 49: 1325-8.
[8] Hodgson, P. D., Aich, P., Manuja, A., Hokamp, K., Roche, F. M., Brinkman, F. S. L., et al. 2005. “Effect of Stress on Viral-Bacterial Synergy in Bovine Respiratory Disease: Novel Mechanisms to Regulate Inflammation.” In Comparative and Functional Genomics, 244-50.
[9] Wilson, B. A., and Ho, M. 2013. “Pasteurella multocida: From Zoonosis to Cellular Microbiology.” Clin Microbiol Rev. 26: 631-55.
[10] Wilkie, I. W., Harper, M., Boyce, J. D., and Adler, B. 2012. “Pasteurella multocida: Diseases Andpathogenesis.” Curr Top Microbiol Immunol. 361: 1-22.
[11] Mutters, R., Ihm, P., Pohl, S., Frederiksen, W., and Mannheim, W. 1984. “Reclassification of the Genus Pasteurella on the Basis of DNA Homology with Proposals for the New Species Pasteurella-Dagmatis New-Species and Pasteurella-Canis Pasteurella-Stomatis New-Species Pasteurella-Anatis New-Species Pasteurella-Langaa New-Species.” Int J Syst Bacteriol.
[12] Carter, G. R. 1955. “Studies on Pasteurella multocida. I. A Hemagglutination Test for the Identification of Serological Types.” Am J Vet Res. 16: 481-4.
[13] Marza, A., Abdullah, F., Ahmed, I., Chung, E., Ibrahim, H., ZamriSaad, M., et al. 2015. “Involvement of Nervous System in Cattle and Buffaloes due to Pasteurella multocida B:2 Infection: A Review of Clinicopathological and Pathophysiological Changes.” J Adv Vet Anim Res. 2: 252. doi: 10.5455/javar.2015.b99.
[14] Harper, M., Boyce, J. D., and Adler, B. 2006. “Pasteurella multocida Pathogenesis: 125 Years after Pasteur.” FEMS Microbiology Letters 265: 1-10.
[15] Berhe, K., Weldeselassie, G., Bettridge, J., Christley, R. M., and Abdi, R. D. 2017. “Small Ruminant pasteurellosis in Tigray Region, Ethiopia: Marked Serotype Diversity May Affect Vaccine Efficacy.” Epidemiol Infect. 145: 1326-38.
[16] Jaglic, Z., Kucerova, Z., Nedbalcova, K., Hlozek, P., and Bartos, M. 2004. “Identification of Pasteurella multocida Serogroup F Isolates in Rabbits.” J Vet Med Ser B Infect Dis Vet Public Heal. 51: 467-9.
[17] Sebbar, G., Zro, K., Kichou, F., Fillali, M. A., and Belkadi, B. 2018. “Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida Species from Ruminants in Six Different Regions in Morocco.” J Agric Sci Technol. 8: 387-94.
[18] Ryan, K. A., and Lo, R. Y. C. 1999. “Characterization of a CACAG Pentanucleotide Repeat in Pasteurella haemolytica and Its Possible Role in Modulation of a Novel Type III Restriction-Modification System.” Nucleic Acids Res. 27: 1505-11.
[19] Dassanayake, R. P., Call, D. R., Sawant, A. A., Carol Casavant, N., Weiser, G. C., Knowles, D. P., et al. 2010. “Bibersteinia Trehalosi Inhibits the Growth of Mannheimia haemolytica by a Proximity-Dependent Mechanism.” Appl Environ Microbiol. 76: 1008-13.
[20] Townsend, K. M., Boyce, J. D., Chung, J. Y., Frost, A. J., and Adler, B. 2001. “Genetic Organization of Pasteurella multocida Cap Loci and Development of a Multiplex Capsular PCR Typing System.” J Clin Microbiol. 39: 924-9.
[21] Davies, R. L., Paster, B. J., and Dewhirst, F. E. 2009. “Phylogenetic Relationships and Diversity within the Pasteurella haemolytica Complex Based on 16S rRNA Sequence Comparison and Outer Membrane Protein and Lipopolysaccharide Analysis.” Int J Syst Bacteriol 46: 736-44.
[22] Korczak, B., Christensen, H., Emler, S., Frey, J., and Kuhnert, P. 2004. “Phylogeny of the Family Pasteurellaceae Based on rpoB Sequences.” Int J Syst Evol Microbiol. 54: 1393-9.
[23] Kumar, S., Stecher, G., and Tamura, K. 2016. “MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.” Mol Biol Evol. 33: 1870-4.
[24] Davies, R. L. 2004. “Genetic Diversity among Pasteurella multocida Strains of Avian, Bovine, Ovine and Porcine Origin from England and Wales by Comparative Sequence Analysis of the 16S rRNA Gene.” Microbiology 150: 4199-210.
[25] Sethi, M., Kumar, S., Tomar, N., John, J. K., Das, T., Agarwal, R. K., et al. 2017. “Molecular Diagnosis and Antibiogram of Pasteurella multocida Type B Associated with Septicemic Pasteurellosis in Pigs.” Indian J Anim Sci. 87: 1488-91.
[26] Du, H., Fang, R., Pan, T., Li, T., Li, N., He, Q., et al. 2016. “Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates.” Int J Genomics. https://www.hindawi.com/journals/ijg/.
[27] Varga, Z., Volokhov, D. V., Stipkovits, L., Thumaákos, Sellyei, B., and Magyar, T. 2013. “Characterization of Pasteurella multocida Strains Isolated from Geese.” Vet Microbiol. 163: 149-56.
[28] Besser, T. E., Highland, M. A., Baker, K., Cassirer, E. F., Anderson, N. J., Ramsey, J. M., et al. 2012. “Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008-2010.” Emerg Infect Dis. 18 (3): 406-14.
[29] Weiser, C., Delong, J., Paz, L., Shaffi, B., Price, W., and Ward, C. 2003. “Characterization of Pasteurella multocida Associated with Pneumonia in Bighorn Sheep.” J Wildl Dis. 39: 536-44.
[30] Abadie, G., and Thiery, R. 2008. “Serological Characterisation of Mannheimia haemolytica and Pasteurella trehalosi Strains from Nasal Pits of Domestic Sheep and Goats in the Alpes-Maritimes Department (France).” Rev Med Vet. 159: 101-6.
[31] Blanco-Viera, F. J., Trigo, F. J., Jaramillo-Meza, L., and Aguilar-Romero, F. 1995. “Serotypes of Pasteurella multocida and Pasteurella haemolytica Isolated from Pneumonic Lesions in Cattle and Sheep from México.” Rev Latinoam Microbiol. 37 (2): 121-6.
[32] Kumar, A. A., Shivachandra, S. B., Biswas, A., Singh, V. P., Singh, V. P., and Srivastava, S. K. 2004. “Prevalent Serotypes of Pasteurella multocida Isolated from Different Animal and Avian Species in India.” Vet Res Commun. 28 (8): 657-67.
[33] Sahay, S., Natesan, K., Prajapati, A., Kalleshmurthy, T., Shome, B. R., Rahman, H., et al. 2020. “Prevalence and Antibiotic Susceptibility of Mannheimia haemolytica and Pasteurella multocida Isolated from Ovine Respiratory Infection: A Study from Karnataka, Southern India.” Vet World 13 (9): 1947-54.
[34] Azizi, S., Korani, F. S., and Oryan, A. 2013. “Pneumonia in Slaughtered Sheep in South-Western Iran: Pathological Characteristics and Aerobic Bacterial Aetiology.” Vet Ital. 49 (1):109-18.
[35] Kaoud, H., El-Dahshan, A. R., Zaki, M. M., and Nasr, S. A. 2010. “Occurrence of Mannheimia haemolytica and Pasteurella trehalosi among Ruminants in Egypt.” New York Sci J. 3: 5.
[36] Assefa, G. A., and Kelkay, M. Z. 2018. “Goat Pasteurellosis: Serological Analysis of Circulating Pasteurella Serotypes in Tanqua Aberegelle and Kola Tembien Districts, Northern Ethiopia.” BMC Res Notes. 11: 485.
[37] Legesse, A., Abayneh, T., Mamo, G., Gelaye, E., Tesfaw, L., Yami, M., et al. 2018. “Molecular Characterization of Mannheimia haemolytica Isolates Associated with Pneumonic Cases of Sheep in Selected Areas of Central Ethiopia.” BMC Microbiol. 18: 205.
[38] Doutre, M., and Perreau, P. 1983. “Le portage de Pasteurella sp. et de Mycoplasma arginini chez la chèvre au Sénégal.” Rev d"elevage Med Vet des pays Trop. 36: 11-4. (in French)
[39] Doutre, M., and Perreau P. 1984. “Le portage des Pasteurella sp. et de Mycoplasma arginini chez les moutons sains au Sénégal.” Res Vet Sci. 34: 365-8. (in French)
[40] Martrenchar, A., Zoyem, N., Ngangnou, A., Bouche, F. D., Ngo Tama, A. C., and Njoya, A. 1995. “Etude des principaux agents infectieux intervenant dans l’étiologie des pneumopathies des petits rumin.ants au Nord-Cameroun.” Rev d"elevage Med Vet des pays Trop. 48: 133-7. (in French)
[41] Hussein, A. M., and Elsawi Mohamed, O. 1984. “A Serological Survey of Sheep Sera for Antibodies to Pasteurella haemolytica Serotypes in the Sudan.” Rev d"elevage Med Vet des pays Trop. 37: 418-21.
[42] Dabo, S. M., Taylor, J. D., and Confer, A. W. 2008. “Pasteurella multocida and Bovine Respiratory Disease.” Animal Health Research Reviews 8: 129-50.
[43] Król, J., Bania, J., Florek, M., Podkowik, M., Pliszczak-Król, A, and Staroniewicz, Z. 2011. “Genetic Diversity of Pasteurella dagmatis as Assessed by Analysis of the 16S rRNA and rpoB Gene Sequences.” Curr Microbiol. 63: 87-93.
[44] Welsh, R. D., Dye, L. B., Payton, M. E., and Confer, A. W. 2004. “Isolation and Antimicrobial Susceptibilities of Bacterial Pathogens from Bovine Pneumonia: 1994-2002.” J Vet Diagnostic Investig. 16: 426-31.
[45] Dey, S., Singh, V. P., Kumar, A. A., Sharma, B., Srivastava, S. K., and Singh N. 2007. “Comparative Sequence Analysis of 16S rRNA Gene of Pasteurella multocida Serogroup B Isolates from Different Animal Species.” Res Vet Sci. 83: 1-4.
[46] Peng, Z., Liang, W., Wang, F., Xu, Z., Xie, Z., Lian, Z., et al. 2018. “Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates from Different Host Species.” Front Microbiol. 9: 1408.
[47] Davies, R. L., MacCorquodale, R., and Reilly, S. 2004. “Characterisation of Bovine Strains of Pasteurella multocida and Comparison with Isolates of Avian, Ovine and Porcine Origin.” Vet Microbiol. 99: 145-58.
[48] Ramdani, H. J. S. D., Johnson, R. B., Spencer, T. L., and Adler, B. 1990. “Pasteurella multocida Infections in Mice with Reference to Haemorrhagic Septicaemia in Cattle and Buffalo.” Immunol Cell Biol. 68: 57-61.
[49] Heddleston, K. L., Rhoades, K. R., and Rebers, P. A. 1967. “Experimental Pasteurellosis: Comparative Studies on Pasteurella multocida from Asia, Africa, and North America.” Am J Vet Res. 28: 1003-12.
[50] Waffa, A. A., Al-Gebouri, N., and Al-Maaly, N. M. 2014. “Study the Pathogenicity of Pasteurella multocida in Mice.” J. Vet Res. 1: 312-23.
[51] Hussaini, J., Nazmul, M. H. M., Masyitah, N., Abdullah, M. A., and Ismail, S. 2013. “Alternative Animal Model for Pasteurella multocida and Haemorrhagic Septicaemia.” Biomed Res. 24: 263-6.
[52] Mahmood, A. A., Sidik, K., Salmah, I., and Suzainur, K. 2004. “Effects of Dexamethasone on Rats Infected with Pasteurella multocida.” J Anim Vet Adv. 3: 39-44.
[53] Zamri-Saad, M., Effendy, A. W. M., Maswati, M. A., Nsali, M., and Sheikh-Omar, A. R. 1996. “The Goat as a Model for Studies of Pneumonic pasteurellosis Caused by Pasteurella multocida.” British Veterinary Journal 152: 453-8.