Affiliation(s)
1. Universidad Colegio Mayor de Cundinamarca, Facultad de Ciencias de la Salud, Programa de Bacteriología, Calle 28 No. 5B-02, Bogotá, Colombia
2. Universidad de la Sabana, Facultad de Ingeniería, Programa de Doctorado en Biociencias, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia
ABSTRACT
The “oil stain disease”
has become a phytosanitary status that involves economic losses for farmers who
produce gulupa (Passiflora edulis Sims).
For this reason, this research aimed to evaluate the biocontrol activity of Bacillus subtilis ATCC 55033 in a
culture medium based on fermented millet as a biocontrol product of the
disease. Initially, metabolite production of seven strains of B. subtilis and a reference strain B.s (ATCC 55033) was evaluated; in addition, the in vitro antagonistic ability was
evaluated against Xanthomonas axonopodis.
Two experiments were performed under in vitro conditions: (1) study of the presence of halos of hemolysis due
to metabolite production; (2) analysis of the antagonistic ability by using
double layer. It was observed that concerning
the average, a number five times bigger of halos of hemolysis and 10 times
bigger halos of inhibition in vitro on the growth of X. axonopodis in the
strains B.s (ATCC 55033) and B.s TB2 over other strains. Subsequently, the evaluation was performed under
greenhouse conditions, based on a randomized block design,
in which two treatments were applied over the soil: (1) application of the fermented medium based on millet
with B.s ATCC 55033, 24 h before X.a inoculation; (2) application of 5 d
post-inoculation. It was observed that the treatment, previous infective
process, obtained an average percentage of severity equal to 42.044%. In addition, after the evaluation month, a
foliage restoration was presented. In conclusion, it is suggested that these products could act as inducers
in the plant systemic resistance to trigger defense responses to infection of X.a.
KEYWORDS
Xanthomonas, Bacillus, biocontrol, bacterioses.
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