Paper Status Tracking
Contact us
[email protected]
Click here to send a message to me 3275638434
Paper Publishing WeChat

Article
Affiliation(s)

1. ICAAM–Mediterranean Institute of Agronomical and Environmental Sciences, Institute for Advanced Research and Formation, Évora University, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal
2. Plant Molecular Biology and Biotechnology Laboratory, Unit of Molecular Biology and Plant Biotechnology (BioFIG), FCUL Campus, C2.2.12, Campo Grande 1749-016, Lisboa, Portugal
3. Plant Science Department, ICAAM, Science and Technology School, Évora University, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal

ABSTRACT

Trincadeira and Aragonez are two important grapevine cultivars in the Iberian Peninsula, used for high quality red wines production. Both cultivars are strongly affected by fungal diseases, with consequent high loses on plant productivity and fruit quality. A successful protocol for plant regeneration via somatic embryogenesis (SE) was established for both cultivars allowing further plant improvement based on gene transfer technology. Several factors were evaluated during the three different phases which characterize an SE plant regeneration protocol. The culture room temperature during the induction phase, a parameter usually accepted as standard by most researchers, proved in these trials to significantly affect the embryo induction rates. Concerning embryo conversion, it was specially affected by the embryo developmental stage, by the intensity and duration of the chilling treatment and by the supplementation of conversion culture medium with activated charcoal. The responses obtained, both for induction and conversion, proved to be highly genotype dependent. Calli structure, as well as embryo integrity, was histologically observed, allowing to characterize embryonic and non-embryonic masses and to identify abnormalities on embryo development.

KEYWORDS

Aragonez, Trincadeira, grapevine, megasporogenesis, somatic embryogenesis.

Cite this paper

References

About | Terms & Conditions | Issue | Privacy | Contact us
Copyright © 2001 - David Publishing Company All rights reserved, www.davidpublisher.com
3 Germay Dr., Unit 4 #4651, Wilmington DE 19804; Tel: 1-323-984-7526; Email: [email protected]