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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
The Cytochrome b Polymorphism of Meat Lines Rabbits
Vladimir Parkanyi, Lubomir Ondruska and Dusan Vasicek
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DOI:10.17265/1934-7391/2016.01.003
The cytochrome b mtDNA was analysed from peripheral whole blood samples of meat lines rabbits. The PCR product—692 bp long fragment of Oryctolagus cuniculus_cytochrome b (OCU07566 GenBank, NCBI, USA) was amplified. Synthetically produced oligonucleotides for the detection of cyt b were designed by own algorithm: ORYCTO-cyt b-FOR 5'- CTA TCA GCA ATC CCA TAT ATC -3' and ORYCTO-cyt b-REV 5'- CTT CAT TTG AGG ATT TTG TT -3'. Based on AluI-RFLP were described two cytochrome b haplotypes—cyt b 430 and cyt b 306. Haplotype cyt b 430 is 571A (190Threonine-T) + 877G (292Alanine-A). The new haplotype cyt b 306 is presented A571G nucleotide substitution, i.e, 571G (190Alanine-A) + 877G (292Alanine-A).
Rabbit, cytochrome b, SNP, new haplotype, A571G