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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Hosna Ara Chowdhury1, Nurul Islam2, Belal Hossain1, Moudood Ahmed1, Sayed Mohsin1 and Rafiqul Islam1
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DOI:10.17265/2161-6256/2015.06.006
Affiliation(s)
1. Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh
2. Department of Botany, University of Dhaka, Dhaka 1000, Bangladesh
ABSTRACT
This study was conducted
to identify a cheap and suitable culture medium for the mycelial growth and
sporulation of Stemphylium vesicarium and to determine the
cultural and morphological variability of this pathogen. A total of 24 isolates of S. vesicarium collected from eight
different onion growing areas were characterized in terms of cultural and
morphological aspects. Front colony colors were greenish brown to dirty white, deep grey to whitish,
light grey to whitish, deep greenish white, light grey and dirty white to
greenish. Reverse colony colors were brown, deep brown and light brown. Colony shapes were circular and irregular with umbonate,
raised and flat type colony elevation. Colony textures were cottony, fluffy and
velvety with entire, undulate and filiform type colony margin. Among the
culture media, V-7 juice agar found to be the most suitable culture media for
mycelial growth of S. vesicarium. The
sporulation of the fungus was remarkably influenced by V-7 juice mixed with potato
dextrose agar (PDA) media, this media
exhibited the highest sporulation (87.76-169.0/mm2)
of S. vesicariumin comparison with other media. The minimum days (28 d to 31 d) for conidial
production were observed on V-7 juice agar medium. The length of conidia varied
from 14.6 µm to 30.6 µm. The maximum mean length
of conidia was 29.97 µm found in isolate DSSA, while the minimum mean length 17.36 µm was found in
isolate MSMM 02. The breadth of conidia ranged from 4.7 µm to 15.7 µm. The maximum mean breadth of
conidia was 12.55 µm found in the isolate DSSA,
while the minimum mean breadth 9.760
µm was found in the isolate CCKH 02. The
horizontal septation varied from 1 to 3 and the longitudinal
septation varied from 0 to 4.
KEYWORDS
Onion, white blotch, S. vesicarium, V-7 juice agar.
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